The Tucker Lab - K+ Channel Research Group




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77. Trick JL, Aryal P, Tucker SJ and Sansom MSP (2015) Molecular simulation studies of hydrophobic gating in nanopores and ion channels.
Biochemical Society Transactions (In Press)

76. Parolin Schnekenberg R, Perkins E, Miller J, Davies WIL, D’Adamo MC, Pessia M, Fawcett K, Sims D, Gillard E, Hudspith K, Williams J, O’Regan M, Jayawant S, Jefferson R, Hughes S, Steinlin M, Lustenberger A, Ragoussis J, Jackson M, Tucker SJ and Németh AH (2015) De novo point mutations in patients diagnosed with ataxic cerebral palsy
Brain  (In Press)

75. Dong YY, Pike ACW, Mackenzie A, McClenaghan C, Aryal P, Dong L, Quigley A, Grieben M, Goubin S, Mukhopadhyay S, Ruda GF, Clausen MV, Cao L, Brennan PE, Burgess-Brown NA, Sansom MSP, Tucker SJ and Carpenter, EP (2015)  K2P channel gating mechanisms revealed by structures of TREK-2 and a complex with Prozac
Science 347: 256-1259 [PDB and PDF]

74. D'Adamo MC, Gallenmüller C, Servettini I, Hartl E, Tucker SJ, Arning L, Biskup S, Grottesi A, Guglielmi L, Imbrici P, Bernasconi P, Di Giovanni G, Franciolini F, Catacuzzeno L, Pessia M, Klopstock T.  (2015) Novel phenotype associated with a mutation in the KCNA1(Kv1.1) gene.
Frontiers in Physiology 5:525. [PDF].

73. Aryal P, Abd-Wahab F, Bucci G, Sansom MSP and Tucker SJ. (2015) Influence of Lipids on the Hydrophobic Barrier within the Pore of the TWIK-1 K2P Channel
Channels 9:44-9  [PDF]

72. Fowler PW, Bollepalli MK, , Rapedius M, Nemetian-Ardestani, E, Shang L, Sansom MSP, Tucker SJ and Baukrowitz T. (2014) Insights into the structural nature of the transition state in the Kir channel gating pathway
Channels 8:551-5  [PDF]

71. Aryal P, Sansom MSP and Tucker SJ. (2014)  Hydrophobic Gating in Ion Channels 
Journal of Molecular Biology  427:121-30 [PDF]

70. Aryal P, Abd-Wahab F, Bucci G, Sansom MSP and Tucker SJ. (2014) A hydrophobic barrier deep within the inner pore of the TWIK-1 K2P potassium channel 
Nature Communications 5:4377   [PDF] [MOVIE]

69. Bollepalli MK, Fowler PW, Rapedius M, Shang L, Sansom MSP, Tucker SJ and Baukrowitz T. (2014) State-Dependent Network Connectivity Determines Gating in a K+ Channel
Structure 22: 1037–1046  [PDF] [Supplemental Files]

68. Veale EL, Al-Moubarak E, Bajaria N, Omoto K, Cao L, Tucker SJ, Stevens EB, Mathie A. (2014) Influence of the N-terminus on the Biophysical Properties and Pharmacology of TREK1 Potassium Channels.
Molecular Pharmacology 85:671-81  [PDF]

67. Zubcevic L, Bavro VN, Muniz JRC,  Schmidt MR, Wang S, De Zorzi R, Vénien-Bryan C, Sansom MSP, Nichols CG and Tucker SJ (2014) Control of KirBac3.1 potassium channel gating at the interface between cytoplasmic domains
Journal of Biological Chemistry  
289:143-51 [PDF]

66. Schmidt MR, Stansfeld PJ, Tucker SJ, and Sansom MSP. (2013)  Simulation-Based Prediction of Phosphatidylinositol 4,5-Bisphosphate Binding to an Ion Channel .
Biochemistry 52:279-81 [PDF]

65. Rapedius M, Schmidt MR, Sharma C, Stansfeld PJ, Sansom MSP,  Baukrowitz T and Tucker SJ. (2012)  State-independent intracellular access of quaternary ammonium blockers to the pore of TREK-1.
Channels 6:473-8 [PDF]

64. Andres-Enguix I, Shang L, Stansfeld PJ, Morahan JM, Sansom MSP, Lafrenière RG, Roy B, Griffiths LR, Rouleau GA, Ebers GC, Cader MZ and Tucker SJ (2012) Functional analysis of missense variants in the TRESK (KCNK18) K+ channel.
Scientific Reports 2:237 [PDF]

63. Bavro VN, De Zorzi R, Schmidt MR, Muniz JRC, Zubcevic L, Sansom MSP, Vénien-Bryan C and Tucker SJ (2012) Structure of a KirBac potassium channel with an open bundle-crossing indicates a mechanism of channel gating
Nature Structural and Molecular Biology 19:158-63 [PDF] [MOVIE 1] [MOVIE 2] [Featured Article] 

62. Paulais M, Bloch-Faure M, Picard N, Jacques T, Ramakrishnan SK, Keck M, Sohet F, Eladari D, Houillier P, Lourdel S, Teulon J and Tucker SJ (2011) Renal phenotype in mice lacking the Kir5.1 (Kcnj16) K+ channel subunit contrasts with that observed in SeSAME/EAST syndrome
Proc. Natl. Acad. Sci. (USA) 108:10361-6

61. Leptihn S, Thompson JR, Ellory JC, Tucker SJ and Wallace MI (2011) In Vitro Reconstitution of Eukaryotic Ion Channels Using Droplet Interface Bilayers.
Journal of the American Chemical Society. 133:9370-5  [PDF] 

60. Piechotta PL, Rapedius M, Stansfeld PJ, Bollepalli MK, Ehrlich G, Andres-Enguix I, Fritzenschaft H, Decher N, Sansom MSP, Tucker SJ and Baukrowitz T. (2011)  The Pore Structure and Gating Mechanism of K2P Channels
EMBO Journal  30: 3607 - 3619  [PDF] [Supplementary PDF]  [MOVIE] [PDB] 

59. Trapp S, Tucker SJ and Gourine AV. (2011) Respiratory responses to hypercapnia and hypoxia in mice with genetic ablation of Kir5.1 (Kcnj16).
Experimental Physiology 96: 451-459  [PDF]

58. D'Adamo MC, Shang L, Imbrici P, Brown SD, Pessia M, Tucker SJ. (2011) Genetic inactivation of Kcnj16 identifies Kir5.1 as an important determinant of neuronal PCO2/pH sensitivity.
Journal of Biological Chemistry 286: 192-198 [PDF]

57. Lafrenière RG, Cader MZ, Poulin JF, Andres-Enguix I, Simoneau M, Gupta N, Boisvert K, Lafrenière F, McLaughlan S, Dubé MP, Ramagopalan S, Ansorge O, Sequeiros J, Pereira-Monteiro JM, Griffiths LR, Tucker SJ, Ebers G and Rouleau GA (2010) A dominant-negative mutation in the TRESK potassium channel (KCNK18) causes familial migraine with aura.
Nature Medicine 16:1157-60 [PDF]  [Supplementary PDF]  [Comment]  [BBC NEWS]

56. Wang SC, Politis A, Di Bartolo N, Bavro VN, Tucker SJ, Booth PJ, Barrera NP and Robinson CV. (2010) Ion mobility mass spectrometry of two tetrameric membrane protein complexes reveals compact structures and differences in stability and packing.
Journal of the American Chemical Society. 132:15468-70 [PDF]  [Supplementary PDF]

55. Paynter JJ, Andres-Enguix I, Fowler PW, Tottey S, Cheng W, Enkvetchakul D, Bavro VN, Kusakabe Y, Sansom MSP, Robinson NJ, Nichols CG and Tucker SJ. (2010) Functional complementation and genetic deletion studies of KirBac channels: activatory mutations highlight gating-sensitive domains.
Journal of Biological Chemistry 285:40754-61 [PDF]  [Supplementary PDF]  [MOVIE]

54. Paynter JJ, Shang L, Bollepalli MK, Baukrowitz T, Tucker SJ. (2010) Random mutagenesis screening indicates the absence of a separate H+-sensor in the pH-sensitive Kir channels.
Channels 4
:390-7 [PDF]

53. Gupta S, Bavro VN, D'Mello R, Tucker SJ, Vénien-Bryan C, Chance MR. (2010) Conformational changes during the gating of a potassium channel revealed by structural mass spectrometry.

[PDF]  [Supplementary PDF]  Selected for Journal Cover  [URL]

52. Shang, L., Ranson, S.V. and  Tucker, S.J. (2009) Long Lived Subconductance Levels in Heteromeric Kir4.1/Kir5.1 Channels from Xenopus tropicalis
Biochemical and Biophysical Research Communications 388:  501–505  [PDF]

51. Imbrici, P., Grottesi, P., D'Adamo, M.C., Tucker, S.J., Mannucci, R., and Pessia, M. (2009) Contribution of the central hydrophobic residue in the PXP motif of voltage-dependent K+ channels to S6 flexibility and gating properties
3:  39-45  [PDF]

50. Imbrici, P., Gualandi, F., D’Adamo, M.C., Taddei Masieri, M., Cudia, P., De Grandis, D., Mannucci, R.,  Nicoletti, I., Tucker, S.J.  Ferlini, A. and Pessia, M. (2008) A Novel KCNA1 Mutation Identified in an Italian Family Affected by Episodic Ataxia Type 1
Neuroscience   157: 577-87 [PDF] Selected for Journal Cover [COVER]

49. Paynter, J.J., Sarkies, P., Andres-Enguix, I., and Tucker, S.J. (2008)  Genetic Selection of Activatory Mutations in KcsA
Channels   2: 413-418 
[PDF]  Selected for Journal Cover [COVER]

48. Nagaoka, Y., Shang, L., Banerjee, A., Bayley, H., and Tucker, S.J. (2008)  Peptide Backbone Mutagenesis of Putative Gating Hinges in a Potassium Ion Channel
ChemBiochem   9: 1725 – 1728  [PDF]

47. Tucker, S.J. and  Baukrowitz, T (2008)  How highly charged anionic lipids bind and regulate ion channels
Journal of General Physiology  131: 431-8 [PDF]

46. Shang, L. and Tucker, S.J. (2008) Non-Equivalent Role of TM2 Gating Hinges in Heteromeric Kir4.1/Kir5.1 Potassium Channels.
European Biophysics Journal  37: 165-171 [PDF]

45.  Rapedius, M., Paynter, J.J., Fowler, P.W, Shang, L., Sansom, MSP., Tucker, SJ and  Baukrowitz, T (2007)  Control of pH and PIP2 gating in heteromeric Kir4.1/Kir5.1 channels by H-bonding at the helix-bundle crossing
Channels 1: 327 - 330  [PDF]

44.  Rapedius, M., Fowler, P.W, Shang, L., Sansom, MSP., Tucker, SJ and Baukrowitz, T (2007)  H-Bonding at the Helix-Bundle Crossing Controls Gating in Kir Potassium Channels
Neuron 55: 602-614  [PDF]  [MOVIE]   See also accompanying 'Preview'  [PDF] 

43.  Haider, S., Khalid, S., Tucker, S.J., Ashcroft, F.M. and Sansom, M.S.P. (2007) Molecular Dynamics Simulations of Inwardly Rectifying (Kir) Potassium Channels: A Comparative Study
Biochemistry  46: 3643-3652  [PDF]

42.  Sun, S., Gan, J.H, Paynter, J.J. and Tucker, S.J.  (2006) Cloning and functional characterisation of a superfamily of microbial inwardly-rectifying (KirBac) potassium channels
Physiological Genomics  26:1-7 [PDF]

41.  Rapedius, M., Haider, S., Browne, K.F., Shang, L., Sansom, M.S.P., Baukrowitz, T. and Tucker, S.J.  (2006) Structural and functional analysis of the putative pH-sensor in the Kir1.1 (ROMK) potassium channel
EMBO Reports  6: 611-616   [PDF]

40.  Rapedius, M., Soom, M., Shumilina, E., Schulze, D., Schönherr, R., Kirsch, C., Lang, F., Tucker, S.J., and Baukrowitz, T. (2005) Long chain CoA esters as competitive antagonists of PIP2 activation in Kir channels
Journal of Biological Chemistry.  280: 30760 – 30767   [PDF]

39.  Shang, L., Lucchese, CJ, Haider, S. and Tucker, SJ. (2005)
  Functional characterisation of missense variations in the Kir4.1 potassium channel (KCNJ10) associated with seizure susceptibility
Molecular Brain Research.  139: 178 – 183  [PDF]

38.  Casamassima, M, D’Adamo, M.C., Pessia, M and Tucker S.J. (2003)  Identification of a heteromeric interaction which influences the rectification, gating and pH-sensitivity of Kir4.1/Kir5.1 potassium channels.
Journal of Biological Chemistry.  278: 43533 – 43540   [PDF]

37.  Konstas, A., Korbmacher, C. and Tucker, S.J. (2002) Identification of domains that control the heteromeric assembly of Kir5.1/Kir4.0 potassium channels

American Journal of Physiology.  284: C910-C917    [PDF]

36.  Konstas, A., Koch, J.P., Tucker, S.J. and Korbmacher, C. (2002) CFTR-dependent upregulation of Kir1.1 (ROMK) renal K+ channels by the epithelial sodium channel (ENaC).
Journal of Biological Chemistry  277: 25377-25384   [PDF]

35.  Konstas, A., Dabrowski, M., Korbmacher, C. and Tucker, S.J. (2002) Intrinsic sensitivity of Kir1.1 (ROMK) to glibenclamide in the absence of SUR2B:  Implications for the identity of the renal ATP-regulated secretory K+ channel
Journal of Biological Chemistry.  277: 21346-21351  [PDF]

34.  Jones, P.A., Tucker, S.J. and Ashcroft, F.M. (2001)  Multiple sites of interaction between the intracellular domains of an inwardly-rectifying potassium channel, Kir6.2.
FEBS Letters . 508:   85-89.  [PDF]

33.  Pessia, M., Imbrici, P., D’Adamo, M.C., Salvatore, L., and Tucker, S.J. (2001)   Differential pH-sensitivity of Kir4.1 and Kir4.2 potassium channels and their modulation by heteropolymerisation with Kir5.1.
Journal of Physiology. 532: 359-367.  [PDF] Selected for Journal Cover [COVER]

32.  Gribble, F.M., Loussouarn, G., Tucker, S.J., Zhao, C., Nichols, C.G., and Ashcroft., F.M. (2000)    A novel method for measurement of sub-membrane ATP-concentration.
Journal of Biological Chemistry. 275: 30046-30049.  [PDF]

31. Imbrici, P., Tucker, S.J., D’Adamo, M.C., and Pessia, M. (2000)  Role of RPTP a- and tyrosine phosphorylation in the serotonergic inhibition of voltage-dependent potassium channels.
European Journal of Physiology. 441: 257-262  [PDF]

30. Tucker, S.J., Imbrici, P., Salvatore, L. and Pessia, M. (2000)  pH-dependence of the inwardly rectifying potassium channel, Kir5.1 and localisation in renal tubular epithelia.

Journal of Biological Chemistry.  275:   16404-16407  [PDF]

29. Tanabe, K., Tucker, S.J.,  Ashcroft, F.M., Proks, P., Kioka, N., Amachi, T., and Ueda, K. (2000) Direct Photoaffinity Labeling of Kir6.2 by [ g-32P]ATP-[ g]4-Azidoanilide.
Biochemical and Biophysical Research Communications272: 316-319  [PDF]

28. Tucker, S.J. and Ashcroft, F.M. (1999)  Mapping of the physical interaction between the intracellular domains of an inwardly-rectifying potassium channel, Kir6.2.
Journal of Biological Chemistry. 274: 33393-33397.  [PDF]

27. Matsuo, M, Tucker, SJ, Ashcroft, FM, Amachi, T., and Ueda, K. (1999)  NEM modification prevents high-affinity ATP-binding to the first nucleotide binding fold of SUR1.
FEBS Letters. 458: 292-294.  [PDF]

26.  Reimann, F., Ryder, T.J., Tucker, S.J., and Ashcroft, F.M. (1999)  An investigation into the role of lysine 185 in the inhibition of Kir6.2 by ATP.
Journal of Physiology. 520: 661-691.  [PDF]

25.  Reimann, F., Tucker, S.J., Proks, P., and Ashcroft, F.M. (1999)  Involvement of the N-terminus of Kir6.2 in coupling to the sulphonylurea receptor
Journal of Physiology.  518: 325-336.  [PDF]

24. Baukrowitz, T., Tucker, S.J., Schulte, U., Benndorf, K., Ruppersberg, J.P., and Fakler, B.   (1999) Inward- rectification in KATP channels: a pH switch in the pore.
EMBO Journal. 18: 847-893.  [PDF]

23. Tanabe, K., Tucker, S.J., Matsuo, M., Proks, P., Ashcroft, F.M., Seino, S., Amachi, T., and Ueda, K (1999)  Photoaffinity labeling of the Kir6.2 subunit of the ATP-sensitive K+ channel by 8-azido-ATP.
Journal of Biological Chemistry.  274 : 3931-3933.  [PDF]

22. Proks, P., Gribble, FM, Adhikari, R., Tucker, S.J., and Ashcroft, F.M. (1999)   Involvement of the N-terminus in the inhibition of the KATP channel by ATP.
Journal of Physiology. 514: 19-25.  [PDF]

21. Baukrowitz, T., Schulte U., Oliver, D., Herlitze, S., Krauter, T., Tucker, S.J., Ruppersberg, J.P. and Fakler, B. (1998)   PIP2  and PIP as determinants for ATP inhibition of KATP channels.
Science.  282: 1141-1141.  [PDF]

20. Trapp, S., Tucker, S.J., and Ashcroft, F.M. (1998) Mechanism of KATP channel inhibition by sulphydryl modifying agents.
Journal of General Physiology. 112: 325-332.  [PDF] Selected for Journal Cover [URL]
19. Trapp, S., Proks, P., Tucker, S.J., and Ashcroft, F.M. (1998) Molecular analysis of KATP channel gating and implications for channel inhibition by ATP.
Journal of General Physiology 112: 333-349.  [PDF]

18. Tucker, S.J., Gribble, F.M., Proks, P., Trapp, S., Ryder, T.J., Reimann, F., and Ashcroft, F.M. (1998)   Molecular determinants of KATP channel inhibition by ATP .
EMBO Journal. 17: 3290-3296.  [PDF]

17. Gribble, F.M, Tucker, S.J., Seino, S., and Ashcroft, FM. (1998)   Tissue specificity of sulphonylureas: studies on cloned cardiac and b-cell KATP channels.
Diabetes. 47: 1412-1418.   [PDF]

16.  Takada, Y., Yamada, K., Taguchi, Y., Kino, K., Matsuo, M., Tucker, S.J., Komano, T., and Ueda, K. (1998)   Non-equivalent cooperation between the two nucleotide-binding folds of P-glycoprotein.
Biochim. Biophys. Acta. 1373: 131-136.  [PDF] 

15. Tucker, S.J., and Ashcroft, F.M (1998)  A touching case of channel regulation: studies on the ATP-sensitive  K+ channel.
Current Opinion in Neurobiology 8: 316-320.  [PDF]
14. Gribble, F.M, Tucker, S.J., Haug, T, and Ashcroft, F.M. (1998) MgATP activates the b-cell KATP channel by interaction with the SUR1 subunit.
Proc. Natl. Acad. Sci. (USA).  95: 7185-7190.  [PDF]

13. Singer, T.D., Tucker, S.J, Marshall, W.S., and Higgins, C.F. (1998) A divergent CFTR homolog: highly regulated salt transport in the euryhaline teleost fish Fundulus heteroclitus.
American Journal of Physiology . 43: C715-723.  [PDF]

 12. Tucker, S.J., Gribble, F.M., Zhao, C., Trapp, S, and Ashcroft, F.M. (1997)   Truncation of Kir6.2 produces ATP-sensitive K+ channels in the absence of the sulphonylurea receptor.
Nature . 387: 179-183.  [PDF]

11. Trapp, S, Tucker, S.J, and Ashcroft, F.M. (1997) Activation and inhibition of KATP currents by guanine nucleotides is mediated by different channel subunits.
Proc. Natl. Acad. Sci. (USA)  94: 8872-8877.  [PDF]
10. Gribble, F.M, Tucker, S.J, and Ashcroft, F.M. (1997) The interaction of nucleotides with the tolbutamide block of cloned ATP-sensitive K+ channel currents expressed in Xenopus oocytes: a reinterpretation.
Journal of Physiology . 504: 35-45.  [PDF]
9.  Gribble, F.M., Tucker, S.J., and Ashcroft, F.M. (1997)  The essential role of the Walker-A motifs of SUR1 in KATP channel activation by MgADP and diazoxide.
EMBO Journal . 16:1145-1152.  [PDF]

8. Tucker, S.J., Pessia, M, Moorhouse, A.J., Gribble, F, Ashcroft, FM, Maylie, J., and Adelman J.P. (1996)   Heteromeric channel formation and   Ca-free  media reduce the toxic effect of the weaver   Kir 3.2 allele.

FEBS Letters . 390: 253-257.   [PDF]   

7. Lagrutta, A, Bond, C.T., Xia, X., Pessia, M., Tucker, S.J., and Adelman, J.P. (1996) Inward rectifier K+ channels. Cloning, expression and structure-function studies.

Japanese Heart Journal  37 : 651-60.   [PDF]

6. Tucker, S.J., Pessia, M., and Adelman J.P. (1996). Muscarine-gated K+ channel: subunit stoichiometry and structural domains essential for G-protein stimualtion.
American Journal of Physiology   271: H379-385  [PDF]

5. Pessia, M., Tucker, S.J., Lee, K., Bond, C. and Adelman, J.P. (1996). Subunit positional effects revealed by novel heteromeric inwardly-rectifying K+ channels.
EMBO Journal    15: 2980-2987. [PDF]

4. Tucker, S.J., Bond, C.T., Herson, P., Pessia, M., & Adelman, J.P. (1995). Inhibitory interactions between two inwardly rectifying K+ channels are mediated by the transmembrane domains.

Journal of Biological Chemistry  271: 5866-5870.  [PDF]

3.  Sakura, H., Bond, C.T., Warren-Perry, M., Horsley, S., Kearney, L., Tucker, S.J., Adelman, J.P., Turner, R., and Ashcroft, F.M. (1995). Characterization and variation of a human inwardly-rectifying K+ channel gene (KCNJ6 ): a putative ATP-sensitive K+ channel subunit.
FEBS Letters 
367: 193-197.   [PDF]

2. Tucker, S.J., James, M.R. and Adelman J.P. (1995) Assignment of KATP-1, the cardiac ATP-sensitive potassium channel gene (KCNJ5) , to human chromosome 11q24.
Genomics .
28: 127-128.  [PDF]  

1. Tucker, S.J., Tannahill, D. and Higgins C.F. (1992). Identification and developmental expression of the X. laevis cystic fibrosis transmembrane conductance regulator gene.
Human Molecular Genetics . 1: 77-82.  [PDF]