Temporal pixel multiplexing for simultaneous high-speed, high-resolution imaging.
Nat Methods 7:3 (2010) 209-211
Authors:
Gil Bub, Matthias Tecza, Michiel Helmes, Peter Lee, Peter Kohl
Abstract:
We introduce an imaging modality that, by offsetting pixel-exposure times during capture of a single image frame, embeds temporal information in each frame. This allows simultaneous acquisition of full-resolution images at native detector frame rates and high-speed image sequences at reduced resolution, without increasing bandwidth requirements. We demonstrate this method using macroscopic and microscopic examples, including imaging calcium transients in heart cells at 250 Hz using a 10-Hz megapixel camera.
