Beecroft Institute for Particle Astrophysics and Cosmology

Abstract:

Assessment of cell-mediated toxicity has traditionally been achieved by measuring the specific activity of enriched effector cell populations against antigen-loaded target cells labeled with radioactive isotopes in vitro. Fluorometric techniques are viewed as a promising alternative to the use of radioactive isotopes for these analyses. Direct assessment of cytotoxicity in vivo can be achieved by monitoring survival of injected fluorescent targets relative to a differentially labeled internal control population without specific antigen. We have developed this approach, incorporating the use of multiple target cell populations labeled with different dyes so that cytotoxicity can be assessed against titrated doses of a given antigen, or against a range of different antigens, simultaneously. We show that this assay, referred to as the VITAL assay, can be used to assess cytotoxic activity of CTL and iNKT cells in vivo and in vitro. CTL responses measured in vivo could be correlated with antigen doses used in immunization strategies, and also with the size of specific CTL populations enumerated in the blood with fluorescent MHC/peptide tetramers. The VITAL assay is, therefore, a sensitive technique allowing analysis of complex multi-epitope responses.

Abstract:

The colour-magnitude relation (CMR) of cluster ellipticals has been widely used to constrain their star formation histories (SFHs) and to discriminate between the monolithic and merger paradigms of elliptical galaxy formation. We investigate the elliptical CMR predicted in the merger paradigm by using a LCDM hierarchical merger model. We first highlight sections of the literature which indicate that the traditional use of fixed apertures to derive colours gives a distorted view of the CMR due to the presence of colour gradients in galaxies. Fixed aperture observations make the CMR steeper and tighter than it really is. We then show that the star formation history (SFH) of cluster ellipticals predicted by the model is quasi-monolithic, with over 95 percent of the total stellar mass formed before a redshift of 1. The quasi-monolithic SFH produces a predicted CMR that agrees well at all redshifts with its observed counterpart once the fixed aperture effect is removed. More importantly, we present arguments to show that the elliptical-only CMR can be used to constrain the SFHs of present-day cluster ellipticals only if we believe a priori in the monolithic collapse model. It is not a meaningful tool for constraining the SFH in the merger paradigm, because a progressively larger fraction of the progenitor set of present-day cluster ellipticals is contained in late-type star forming systems at higher redshift, which cannot be ignored when deriving the SFHs. Hence, the elliptical-only CMR is not a useful discriminant between the two competing theories of elliptical galaxy evolution.