Prof Sonia Antoranz Contera

Abstract:

The adsorption of fibrinogen followed by specific fibrinogen anti-fibrinogen reaction from a tris-buffered solution at 37°C onto Au, Ta and Ti surfaces was investigated. The concentrations of fibrinogen used were 1 mg/ml, which were close to that found in blood and 0.03 mg/ml. It was found that very small amount of silver on the gold surface have a large effect on the amount of fibrinogen adsorbed. Globular monomeric and polymeric vitronectin proteins for low concentrations were identified using atomic force microscopy (AFM) in liquid.

Abstract:

The influence of the micropatterning of surfaces (in relation to their spreading and morphology) on the behavior of osteoblasts on different surfaces was investigated. Micropatterns were seen to be etched into silicon plates by photolithograhy and coated with a 250 nm tantalum layer. The samples were analyzed by Cryo-SEM (Scanning Electron Microscopy) (CamScan MaXim 2040). The elongation of cells was observed to be faster on deep line patterns than on shallow line patterns.

Abstract:

Trimeric Achromobacter cycloclastes Cu-containing nitrite reductase (CuNIR) proteins adsorbed on gold and graphite have been studied by ambient STM and in situ AFM. STM resolves them individually and in layers, distinguishing the sub-molecular individual units of the trimer. The Cu atoms are not visible to STM. STM shows that individual CuNIR denatures as it adsorbs on Au, although a deformed trimeric shape can be identified in some cases. CuNIR forms disordered layers on gold. On graphite, ordered self-assembled layers of CuNIR have been resolved by in situ AFM and ambient STM forming parallel rows whose separation distance corresponds to the size of one of the units of the trimer, 5nm. Ambient STM can achieve better resolution than in situ AFM in the images of the layers. We observe differences between domains showing the parallel row structure and unstructured parts of the CuNIR layer by in situ phase imaging AFM.