Recognition of different DNA sequences by a DNA-binding protein alters protein dynamics differentially.
FEBS letters 586:3 (2012) 258-262
Abstract:
λ-Repressor-operator sites interaction, particularly O(R)1 and O(R)2, is a key component of the λ-genetic switch. FRET from the dansyl bound to the C-terminal domain of the protein, to the intercalated EtBr in the operator DNA indicates that the structure of the protein is more compact in the O(R)2 complex than in the O(R)1 complex. Fluorescence anisotropy reveals enhanced flexibility of the C-terminal domain of the repressor at fast timescales after complex formation with O(R)1. In contrast, O(R)2 bound repressor shows no significant enhancement of protein dynamics at these timescales. These differences are shown to be important for correct protein-protein interactions. Altered protein dynamics upon specific DNA sequence recognition may play important roles in assembly of regulatory proteins at the correct positions.Regime-Changing Hidden Markov Modeling and Statistical Analysis for Complex Single-Molecule Time Series
Biophysical Journal Elsevier 102:3 (2012) 595a-596a
Single-Molecule DNA Repair in Live Bacteria
Biophysical Journal Elsevier 102:3 (2012) 14a
Single-Molecule FRET in Living Bacteria
Biophysical Journal Elsevier 102:3 (2012) 234a
Single-Molecule Fluorescence Studies of Site-Specific DNA Recombination
Biophysical Journal Elsevier 102:3 (2012) 283a