Gene machines

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Colorful molecular diagnostics.

Clin Chem 58:4 (2012) 659-660

Authors:

Achillefs N Kapanidis, Robert Crawford
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A protein biosensor that relies on bending of single DNA molecules.

Chemphyschem 13:4 (2012) 918-922

Authors:

Robert Crawford, Douglas J Kelly, Achillefs N Kapanidis

Abstract:

A "bendy" protein sensor: A DNA-based sensor that uses folded DNA (through DNA kinks) and protein-induced bending to detect DNA-binding proteins is presented. Single-molecule sensing of a transcriptional activator (catabolite activator protein, CAP, which bends its DNA site by 80°) is demonstrated in solution and on surfaces, both in buffers and in cell lysates. The method should allow detection of a wide range of DNA-bending proteins.
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Recognition of different DNA sequences by a DNA‐binding protein alters protein dynamics differentially

FEBS Letters Wiley 586:3 (2012) 258-262

Authors:

Tanumoy Mondol, Subrata Batabyal, Abhishek Mazumder, Siddhartha Roy, Samir Kumar Pal
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Regime-Changing Hidden Markov Modeling and Statistical Analysis for Complex Single-Molecule Time Series

Biophysical Journal Elsevier 102:3 (2012) 595a-596a

Authors:

Kristofer Gryte, Alistair Wardrope, Geraint Evans, Stephan Uphoff, Ludovic Le Reste, Achillefs N Kapanidis
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Single-Molecule DNA Repair in Live Bacteria

Biophysical Journal Elsevier 102:3 (2012) 14a

Authors:

Stephan Uphoff, Rodrigo Reyes-Lamothe, David J Sherratt, Achillefs N Kapanidis
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