MODULATION OF PROTO-ONCOGENE EXPRESSION BY POLYCHLORINATED BIPHENYLS IN 3T3-L1 CELL LINE
Journal of Toxicology and Environmental Health Part A Taylor & Francis 55:2 (1998) 121-131
Isolation and characterization of RAD51C, a new human member of the RAD51 family of related genes
Nucleic Acids Research Oxford University Press (OUP) 26:5 (1998) 1179-1184
In Vitro Studies on the Metabolism and Toxicity of Aflatoxin B1 in Primary Cultures of Black Catfish (Ictalurus melas) Hepatocytes
Alternatives to Laboratory Animals SAGE Publications 26:2 (1998) 225-239
Cryopreservation of isolated trout hepatocytes: Viability and function in primary culture
Cryo-Letters 19:1 (1998) 55-64
Abstract:
Isolated trout hepatocytes were frozen using a programmable freezer and stored in liquid nitrogen. Satisfactory viability were obtained in cryopreserved hepatocytes (CP) as judged by trypan blue exclusion (TB). The viability of primary cultures of CP hepatocytes, was compared with fresh cells using attachment efficiency (using TB), the rate of neutral red uptake (NRU), metabolism of the tetrazolium salt (MTT), and measurement of intracellular lactate dehydrogenase content (LDH). These results show that the activities of CP cells was lower than in fresh cultures, but remained constant over 72 hr of culture (~70%). The CP cultures retain the aspects of liver-specific function as shown by the induction of cytochrome P-4501A1 (CYP1A1) by 3-methylcholantrene (3-MC) and Benzo[a]pyrene (B[a]P) exposure.Suppression of apoptosis by overexpression of Bcl-2 or Bcl-xL promotes survival and mutagenesis after oxidative damage
Biochimie Elsevier 79:9-10 (1997) 613-617