Cryopreservation of isolated trout hepatocytes: Viability and function in primary culture
Cryo-Letters 19:1 (1998) 55-64
Abstract:
Isolated trout hepatocytes were frozen using a programmable freezer and stored in liquid nitrogen. Satisfactory viability were obtained in cryopreserved hepatocytes (CP) as judged by trypan blue exclusion (TB). The viability of primary cultures of CP hepatocytes, was compared with fresh cells using attachment efficiency (using TB), the rate of neutral red uptake (NRU), metabolism of the tetrazolium salt (MTT), and measurement of intracellular lactate dehydrogenase content (LDH). These results show that the activities of CP cells was lower than in fresh cultures, but remained constant over 72 hr of culture (~70%). The CP cultures retain the aspects of liver-specific function as shown by the induction of cytochrome P-4501A1 (CYP1A1) by 3-methylcholantrene (3-MC) and Benzo[a]pyrene (B[a]P) exposure.Suppression of apoptosis by overexpression of Bcl-2 or Bcl-xL promotes survival and mutagenesis after oxidative damage
Biochimie Elsevier 79:9-10 (1997) 613-617
Metabolism Of Benzo[a]Pyrene in Fish Hepatocytes Cultured on Microplates
Polycyclic Aromatic Compounds Taylor & Francis 11:1-4 (1996) 91-98
Synthesis and properties of oligonucleotides containing the mutagenic base O4-benzylthymidine
Bioorganic & Medicinal Chemistry Elsevier 3:1 (1995) 101-108
1,N6-ethenoadenine is preferred over 3-methyladenine as substrate by a cloned human N-methylpurine-DNA glycosylase (3-methyladenine-DNA glycosylase).
Biochemistry American Chemical Society (ACS) 33:7 (1994) 1624-1628