Prof Christopher Ramsey

Abstract:

AbstractTo achieve a reliable radiocarbon (14C) date for an object, any contamination that may be of a different age must be removed prior to dating. Samples that have been conserved with treatments such as adhesives, varnishes or consolidants can pose a particular challenge to14C dating. At the Oxford Radiocarbon Accelerator Unit (ORAU), common examples of such substances encountered include shellac, the acrylic polymers Paraloid B-67 and B-72, and vinyl acetate-derived polymers (e.g. PVA). Here, a non-carbon-containing absorbent substrate called Chromosorb®was deliberately contaminated with a range of varieties or brands of these conservation treatments, as well as two cellulose nitrate lacquers. A selection of chemical pretreatments was tested for their efficiency at removing them. While the varieties of shellac and Paraloid tested were completely removed with some treatments (water/methanol and acetone/methanol/chloroform sequential washes, respectively), no method was found that was capable of completely removing any of the vinyl acetate-derived materials or the cellulose nitrate lacquers. While Chromosorb is not an exact analog of archaeological wood or bone, for example, this study suggests that it may be possible to remove aged shellac and Paraloid from archaeological specimens with standard organic solvent-acid-base-acid pretreatments, but it may be significantly more difficult to remove vinyl acetate-derived polymers and cellulose nitrate lacquers sufficiently to provide reliable14C dates. The four categories of conservation treatment studied demonstrate characteristic FTIR spectra, while highlighting subtle chemical and molecular differences between different varieties of shellac, Paraloid and cellulose nitrate lacquers, and significant differences between the vinyl acetate derivatives.

Abstract:

Abstract

Abstract:

The ¹³C/¹²C ratio of C₃ plant matter is thought to be controlled by the isotopic composition of atmospheric CO₂ and stomatal response to environmental conditions, particularly mean annual precipitation (MAP). The effect of CO₂ concentration on ¹³C/¹²C ratios is currently debated, yet crucial to reconstructing ancient environments and quantifying the carbon cycle. Here we compare high-resolution ice core measurements of atmospheric CO₂ with fossil plant and faunal isotope records. We show the effect of pCO₂ during the last deglaciation is stronger for gymnosperms (-1.4 ± 1.2‰) than angiosperms/fauna (-0.5 ± 1.5‰), while the contributions from changing MAP are -0.3 ± 0.6‰ and -0.4 ± 0.4‰, respectively. Previous studies have assumed that plant ¹³C/¹²C ratios are mostly determined by MAP, an assumption which is sometimes incorrect in geological time. Atmospheric effects must be taken into account when interpreting terrestrial stable carbon isotopes, with important implications for past environments and climates, and understanding plant responses to climate change.

Abstract:

The melting of perennial ice patches globally is uncovering a fragile record of alpine activity, especially hunting and the use of mountain passes. When rescued by systematic fieldwork (glacial archaeology), this evidence opens an unprecedented window on the chronology of high-elevation activity. Recent research in Jotunheimen and surrounding mountain areas of Norway has recovered over 2000 finds—many associated with reindeer hunting (e.g. arrows). We report the radiocarbon dates of 153 objects and use a kernel density estimation (KDE) method to determine the distribution of dated events fromca4000 BCE to the present. Interpreted in light of shifting environmental, preservation and socio-economic factors, these new data show counterintuitive trends in the intensity of reindeer hunting and other high-elevation activity. Cold temperatures may sometimes have kept humans from Norway's highest elevations, as expected based on accessibility, exposure and reindeer distributions. In times of increasing demand for mountain resources, however, activity probably continued in the face of adverse or variable climatic conditions. The use of KDE modelling makes it possible to observe this patterning without the spurious effects of noise introduced by the discrete nature of the finds and the radiocarbon calibration process.

Abstract:

Rationale

For radiocarbon results to be accurate, samples must be free of contaminating carbon. Sample pre-treatment using an HPLC approach has been developed at the Oxford Radiocarbon Accelerator Unit (ORAU) as an alternative to conventional methods for dating heavily contaminated bones. This approach isolates hydroxyproline from bone collagen, enabling a purified bone-specific fraction to then be radiocarbon dated by accelerator mass spectrometry (AMS).

Methods

Using semi-preparative chromatography and non-carbon based eluents, this technique enables the separation of underivatised amino acids liberated by hydrolysis of extracted bone collagen. A particular focus has been the isolation of hydroxyproline for single compound AMS dating since this amino acid is one of the main contributors to the total amount of carbon in mammalian collagen. Our previous approach, involving a carbon-free aqueous mobile phase, required a 2-step separation using two different chromatographic columns.2

Results

This paper reports significant improvements that have been recently made to the method to enable faster semi-preparative separation of hydroxyproline from bone collagen, making the method more suitable for routine radiocarbon dating of contaminated and/or poorly preserved bone samples by AMS. All steps of the procedure, from the collagen extraction to the correction of the AMS data, are described.

Conclusions

The modifications to the hardware and to the method itself have reduced significantly the time required for the preparation of each sample. This makes it easier for other radiocarbon facilities to implement and use this approach as a routine method for preparing contaminated bone samples.