Beecroft Institute for Particle Astrophysics and Cosmology

Abstract:

Assessment of cell-mediated toxicity has traditionally been achieved by measuring the specific activity of enriched effector cell populations against antigen-loaded target cells labeled with radioactive isotopes in vitro. Fluorometric techniques are viewed as a promising alternative to the use of radioactive isotopes for these analyses. Direct assessment of cytotoxicity in vivo can be achieved by monitoring survival of injected fluorescent targets relative to a differentially labeled internal control population without specific antigen. We have developed this approach, incorporating the use of multiple target cell populations labeled with different dyes so that cytotoxicity can be assessed against titrated doses of a given antigen, or against a range of different antigens, simultaneously. We show that this assay, referred to as the VITAL assay, can be used to assess cytotoxic activity of CTL and iNKT cells in vivo and in vitro. CTL responses measured in vivo could be correlated with antigen doses used in immunization strategies, and also with the size of specific CTL populations enumerated in the blood with fluorescent MHC/peptide tetramers. The VITAL assay is, therefore, a sensitive technique allowing analysis of complex multi-epitope responses.

Abstract:

We present results of a Gemini adaptive optics (AO) imaging program to investigate the host galaxies of typical QSOs at z~2. Our aim is to study the host galaxies of typical, L*_qso QSOs at the epoch of peak QSO and star formation activity. The large database of faint QSOs provided by the 2dF QSO Redshift Survey allows us to select a sample of QSOs at z=1.75-2.5 which have nearby (<12 arcsecond separation) bright stars suitable for use as AO guide stars. We have observed a sample of 9 QSOs. The images of these sources have AO corrected full-width at half-maximum of between 0.11 and 0.25 arcseconds. We use multiple observations of point spread function (PSF) calibration star pairs in order to quantify any uncertainty in the PSF. We then factored these uncertainties into our modelling of the QSO plus host galaxy. In only one case did we convincingly detect a host (2QZ J133311.4+001949, at z=1.93). This host galaxy has K=18.5+-0.2 mag with a half-light radius, r_e=0.55+-0.1'', equivalent to ~3L*_gal assuming a simple passively evolving model. From detailed simulations of our host galaxy modelling process, we find that for four of our targets we should be sensitive to host galaxies that are equivalent to ~2L*_gal (passively evolved). Our non-detections therefore place tight constraints on the properties of L*_qso QSO host galaxies, which can be no brighter (after allowing for passive evolution) than the host galaxies of L*_qso AGN at low redshift, although the QSOs themselves are a factor of ~50 brighter. This implies that either the fueling efficiency is much greater at high redshift, or that more massive black holes are active at high redshift.

Abstract:

The colour-magnitude relation (CMR) of cluster ellipticals has been widely used to constrain their star formation histories (SFHs) and to discriminate between the monolithic and merger paradigms of elliptical galaxy formation. We investigate the elliptical CMR predicted in the merger paradigm by using a LCDM hierarchical merger model. We first highlight sections of the literature which indicate that the traditional use of fixed apertures to derive colours gives a distorted view of the CMR due to the presence of colour gradients in galaxies. Fixed aperture observations make the CMR steeper and tighter than it really is. We then show that the star formation history (SFH) of cluster ellipticals predicted by the model is quasi-monolithic, with over 95 percent of the total stellar mass formed before a redshift of 1. The quasi-monolithic SFH produces a predicted CMR that agrees well at all redshifts with its observed counterpart once the fixed aperture effect is removed. More importantly, we present arguments to show that the elliptical-only CMR can be used to constrain the SFHs of present-day cluster ellipticals only if we believe a priori in the monolithic collapse model. It is not a meaningful tool for constraining the SFH in the merger paradigm, because a progressively larger fraction of the progenitor set of present-day cluster ellipticals is contained in late-type star forming systems at higher redshift, which cannot be ignored when deriving the SFHs. Hence, the elliptical-only CMR is not a useful discriminant between the two competing theories of elliptical galaxy evolution.