Chapter 5

Chapter in RNA Polymerases as Molecular Motors, Royal Society of Chemistry (RSC) (2009) 115-156

Authors:

Achillefs N Kapanidis, Shimon Weiss

Single-Molecule Analysis of Transcription

Biophysical Journal Elsevier 96:3 (2009) 202a

Authors:

Richard Ebright, Shimon Weiss, Anirban Chakraborty, Dongye Wang, You Korlann, Achillefs Kapanidis, Emmanuel Margeat

Single-Molecule Analysis of Transcription

BIOPHYSICAL JOURNAL 96:3 (2009) 202A-202A

Authors:

Richard Ebright, Shimon Weiss, Anirban Chakraborty, Dongye Wang, You Korlann, Achillefs Kapanidis, Emmanuel Margeat

Single-Molecule FRET: Methods and Biological Applications

Chapter in Handbook of Single-Molecule Biophysics, Springer Nature (2009) 129-163

Authors:

Ling Chin Hwang, Johannes Hohlbein, Seamus J Holden, Achillefs N Kapanidis

Single-molecule FRET analysis of protein-DNA complexes.

Methods Mol Biol 543 (2009) 503-521

Authors:

Mike Heilemann, Ling Chin Hwang, Konstantinos Lymperopoulos, Achillefs N Kapanidis

Abstract:

We present a single-molecule method for studying protein-DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules. An application of this method to the study of a bacterial transcription initiation complex is presented.