Gene machines

Research group

Research theme

Sub department

About

Single-Molecule Analysis of Transcription

Biophysical Journal Elsevier 96:3 (2009) 202a

Authors:

Richard Ebright, Shimon Weiss, Anirban Chakraborty, Dongye Wang, You Korlann, Achillefs Kapanidis, Emmanuel Margeat
More details from the publisher
More details

Single-Molecule Analysis of Transcription

BIOPHYSICAL JOURNAL 96:3 (2009) 202A-202A

Authors:

Richard Ebright, Shimon Weiss, Anirban Chakraborty, Dongye Wang, You Korlann, Achillefs Kapanidis, Emmanuel Margeat
More details from the publisher

Single-Molecule FRET: Methods and Biological Applications

Chapter in Handbook of Single-Molecule Biophysics, Springer Nature (2009) 129-163

Authors:

Ling Chin Hwang, Johannes Hohlbein, Seamus J Holden, Achillefs N Kapanidis
More details from the publisher

Single-molecule FRET analysis of protein-DNA complexes.

Methods Mol Biol 543 (2009) 503-521

Authors:

Mike Heilemann, Ling Chin Hwang, Konstantinos Lymperopoulos, Achillefs N Kapanidis

Abstract:

We present a single-molecule method for studying protein-DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules. An application of this method to the study of a bacterial transcription initiation complex is presented.
More details from the publisher

Red light, green light: probing single molecules using alternating-laser excitation.

Biochem Soc Trans 36:Pt 4 (2008) 738-744

Authors:

Yusdi Santoso, Ling Chin Hwang, Ludovic Le Reste, Achillefs N Kapanidis

Abstract:

Single-molecule fluorescence methods, particularly single-molecule FRET (fluorescence resonance energy transfer), have provided novel insights into the structure, interactions and dynamics of biological systems. ALEX (alternating-laser excitation) spectroscopy is a new method that extends single-molecule FRET by providing simultaneous information about structure and stoichiometry; this new information allows the detection of interactions in the absence of FRET and extends the dynamic range of distance measurements that are accessible through FRET. In the present article, we discuss combinations of ALEX with confocal microscopy for studying in-solution and in-gel molecules; we also discuss combining ALEX with TIRF (total internal reflection fluorescence) for studying surface-immobilized molecules. We also highlight applications of ALEX to the study of protein-nucleic acid interactions.
More details from the publisher
More details