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Identification and developmental expression of the Xenopus laevis cystic fibrosis transmembrane conductance regulator gene.

Hum Mol Genet 1:2 (1992) 77-82

Authors:

SJ Tucker, D Tannahill, CF Higgins

Abstract:

An amphibian homologue of the human cystic fibrosis transmembrane conductance regulator (CFTR) gene has been isolated from Xenopus laevis by polymerase chain reaction (PCR) amplification. The 4455bp sequence encodes a predicted polypeptide of 1485 amino acids which has an overall homology at the amino acid level of 77% identity and 88% similarity with human CFTR. Comparison of these evolutionarily diverse CFTR sequences has structure-function implications. Investigation of the expression of the Xenopus gene during early stages of development (Stages 1-48), using RNAase protection assays and PCR analysis of total Xenopus RNA, shows CFTR mRNA to be present at the very earliest stages of development, including the oocyte and blastula stages, with increasing amounts during subsequent development. The identification of mRNA for a CFTR homologue in the Xenopus oocyte and early stages of development has implications for its biological role.
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A unique lower X-gate in TASK channels traps inhibitors within the vestibule

Authors:

Karin EJ Rödström, Aytuğ K Kiper, Wei Zhang, Susanne Rinné, Ashley CW Pike, Matthias Goldstein, Linus Conrad, Martina Delbeck, Michael Hahn, Heinrich Meier, Magdalena Platzk, Andrew Quigley, David Speedman, Leela Shrestha, Shubhashish MM Mukhopadhyay, Nicola A Burgess-Brown, Stephen J Tucker, Thomas Mueller, Niels Decher, Elisabeth P Carpenter
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CryoEM Structure of the human THIK-1 K2P K+ Channel Reveals a Lower ‘Y-gate’ Regulated by Lipids and Anaesthetics

Authors:

Karin EJ Rödström, Bisher Eymsh, Peter Proks, Mehtab S Hayre, Christian Madry, Anna Rowland, Simon Newstead, Thomas Baukrowitz, Marcus Schewe, Stephen J Tucker
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Defective X-gating caused by de novo gain-of-function mutations in KCNK3 underlies a developmental disorder with sleep apnea

Authors:

Janina Sörmann, Marcus Schewe, Peter Proks, Thibault Jouen-Tachoire, Shanlin Rao, Elena B Riel, Katherine E Agre, Amber Begtrup, John Dean, Maria Descartes, Jan Fischer, Alice Gardham, Carrie Lahner, Paul R Mark, Srikanth Muppidi, Pavel N Pichurin, Joseph Porrmann, Jens Schallner, Kirstin Smith, Volker Straub, Pradeep Vasudevan, Rebecca Willaert, Elisabeth P Carpenter, Karin EJ Rödström, Michael G Hahn, Thomas Müller, Thomas Baukrowitz, Matthew E Hurles, Caroline F Wright, Stephen J Tucker
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Extracellular modulation of TREK-2 activity with nanobodies provides insight into the mechanisms of K2P channel regulation

Authors:

Karin EJ Rödström, Alexander Cloake, Janina Sörmann, Agnese Baronina, Kathryn HM Smith, Ashley CW Pike, Jackie Ang, Peter Proks, Marcus Schewe, Ingelise Holland-Kaye, Simon R Bushell, Jenna Elliott, Els Pardon, Thomas Baukrowitz, Raymond J Owens, Simon Newstead, Jan Steyaert, Elisabeth P Carpenter, Stephen J Tucker
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