Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

Nature Methods Springer Nature 20:4 (2023) 523-535

Authors:

Ganesh Agam, Christian Gebhardt, Milana Popara, Rebecca Mächtel, Julian Folz, Benjamin Ambrose, Neharika Chamachi, Sang Yoon Chung, Timothy D Craggs, Marijn de Boer, Dina Grohmann, Taekjip Ha, Andreas Hartmann, Jelle Hendrix, Verena Hirschfeld, Christian G Hübner, Thorsten Hugel, Dominik Kammerer, Hyun-Seo Kang, Achillefs N Kapanidis, Georg Krainer, Kevin Kramm, Edward A Lemke, Eitan Lerner, Emmanuel Margeat, Kirsten Martens, Jens Michaelis, Jaba Mitra, Gabriel G Moya Muñoz, Robert B Quast, Nicole C Robb, Michael Sattler, Michael Schlierf, Jonathan Schneider, Tim Schröder, Anna Sefer, Piau Siong Tan, Johann Thurn, Philip Tinnefeld, John van Noort, Shimon Weiss, Nicolas Wendler, Niels Zijlstra, Anders Barth, Claus AM Seidel, Don C Lamb, Thorben Cordes

A new twist on PIFE: photoisomerisation-related fluorescence enhancement

(2023)

Authors:

Evelyn Ploetz, Benjamin Ambrose, Anders Barth, Richard Börner, Felix Erichson, Achillefs N Kapanidis, Harold D Kim, Marcia Levitus, Timothy M Lohman, Abhishek Mazumder, David S Rueda, Fabio D Steffen, Thorben Cordes, Steven W Magennis, Eitan Lerner

Deep Antimicrobial Susceptibility Phenotyping (DASP) Training and Evaluation Dataset, and Trained Models.

University of Oxford (2023)

Authors:

Aleksander Zagajewski, Piers Turner, Conor Feehily, Nicole Stoesser, Christoffer Nellaker, Achillefs Kapanidis

Abstract:

Dataset of microscopy images of untreated and treated E.coli lab strains and clinical isolates, and machine learning models trained on them. Corresponding publications: https://doi.org/10.1101/2022.12.08.22283219 Corresponding analysis code: https://github.com/KapanidisLab/Deep-Learning-and-Single-Cell-Phenotyping-for-Rapid-Antimicrobial-Susceptibility-Testing

Overcoming the High Concentration Barrier in Single-Molecule Fluorescence Experiments through Adaptable Fluorogenic ssDNA Label

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 52:SUPPL 1 (2023) S192-S192

Authors:

Mirjam Kummerlin, Achillefs Kapanidis

Single-molecule FRET for virology: 20 years of insight into protein structure and dynamics

Quarterly Reviews of Biophysics Cambridge University Press (CUP) 56 (2023) e3

Authors:

Danielle Groves, Christof Hepp, Achillefs N Kapanidis, Nicole C Robb