Rapid functionalisation and detection of viruses via a novel Ca2+-mediated virus-DNA interaction
Scientific Reports Nature Research 9 (2019) 16219
Confinement-free wide-field ratiometric tracking of single fluorescent molecules
Biophysical Journal Elsevier 117:11 (2019) 2141-2153
Recent Advances in Understanding σ70-Dependent Transcription Initiation Mechanisms.
Journal of molecular biology (2019)
Abstract:
Prokaryotic transcription is one of the most studied biological systems, with relevance to many fields including the development and use of antibiotics, the construction of synthetic gene networks, and the development of many cutting-edge methodologies. Here, we discuss recent structural, biochemical, and single-molecule biophysical studies targeting the mechanisms of transcription initiation in bacteria, including the formation of the open complex, the reaction of initial transcription, and the promoter escape step that leads to elongation. We specifically focus on the mechanisms employed by the RNA polymerase holoenzyme with the housekeeping sigma factor σ70. The recent progress provides answers to long-held questions, identifies intriguing new behaviours, and opens up fresh questions for the field of transcription.Recent advances in understanding σ70-dependent transcription initiation mechanisms
Journal of Molecular Biology Elsevier 431:20 (2019) 3947-3959
Abstract:
Prokaryotic transcription is one of the most studied biological systems, with relevance to many fields including the development and use of antibiotics, the construction of synthetic gene networks, and the development of many cutting-edge methodologies. Here, we discuss recent structural, biochemical, and single-molecule biophysical studies targeting the mechanisms of transcription initiation in bacteria, including the formation of the open complex, the reaction of initial transcription, and the promoter escape step that leads to elongation. We specifically focus on the mechanisms employed by the RNA polymerase holoenzyme with the housekeeping sigma factor σ70. The recent progress provides answers to long-held questions, identifies intriguing new behaviours, and opens up fresh questions for the field of transcription.Real-time analysis of single influenza virus replication complexes reveals large promoter-dependent differences in initiation dynamics
Nucleic Acids Research Oxford University Press 47:12 (2019) 6466-6477