X-ray and neutron scattering

Abstract:

A pronounced enthalpy release occurs around 1.38Tg in the prototypical metal-organic framework glass formed from ZIF-4 [Zn(C3H3N2)2], but there is no sign for any crystallization (i.e., long-range ordering) taking place. The enthalpy release peak is attributed to pore collapse and structural densification.

Abstract:

Nucleic acids can fold into well-defined 3D structures that help determine their function. Knowing precise nucleic acid structures can also be used for the design of nucleic acid-based therapeutics. However, locations of hydrogen atoms, which are key players of nucleic acid function, are normally not determined with X-ray crystallography. Accurate determination of hydrogen atom positions can provide indispensable information on protonation states, hydrogen bonding, and water architecture in nucleic acids. Here, we used neutron crystallography in combination with X-ray diffraction to obtain joint X-ray/neutron structures at both room and cryo temperatures of a self-complementary A-DNA oligonucleotide d[GTGG(C^Se)CAC]₂ containing 2'-SeCH₃ modification on Cyt5 (C^Se) at pH 5.6. We directly observed protonation of a backbone phosphate oxygen of Ade7 at room temperature. The proton is replaced with hydrated Mg²⁺ upon cooling the crystal to 100 K, indicating that metal binding is favored at low temperature, whereas proton binding is dominant at room temperature.