Aligning the μs-ALEX Setup.
Cold Spring Harbor protocols 2015:11 (2015) 1027-1028
Abstract:
To achieve single-molecule sensitivity and thus have the ability to detect single diffusing fluorophores, careful alignment of the microsecond-alternating laser excitation (μs-Alex) setup is crucial. The following protocol describes routine alignment for 2c-ALEX (532 nm/635 nm) with spectral windows G(550-620)R(650-750).Alternating Laser Excitation for Solution-Based Single-Molecule FRET.
Cold Spring Harbor protocols 2015:11 (2015) 979-987
Abstract:
Single-molecule fluorescence resonance energy transfer (smFRET) has been widely applied to the study of fluorescently labeled biomolecules on surfaces and in solution. Sorting single molecules based on fluorescent dye stoichiometry provides one with further layers of information and also enables "filtering" of unwanted molecules from the analysis. We accomplish this sorting by using alternating laser excitation (ALEX) in combination with smFRET measurements; here we describe the implementation of these methodologies for the study of biomolecules in solution.Assembling the μs-ALEX Setup.
Cold Spring Harbor protocols 2015:11 (2015) 1024-1026
Abstract:
This protocol describes the construction of a microsecond-alternating laser excitation (μs-ALEX) using two lasers, a green 532-nm acousto-optically modulated laser and a red 635-nm directly modulated laser.Sample Preparation and Data Acquisition for μs-ALEX.
Cold Spring Harbor protocols 2015:11 (2015) 1029-1031
Abstract:
This protocol describes the preparation of samples and data acquisition for microsecond-alternating laser excitation. Sample preparation requires a dilution that ensures the detection of single events.New technologies for DNA analysis – a review of the READNA Project
JournalName Elsevier 33:3 (2015) 311-330