A facile method for reversibly linking a recombinant protein to DNA.
Chembiochem 10:9 (2009) 1551-1557
Abstract:
We present a facile method for linking recombinant proteins to DNA. It is based on the nickel-mediated interaction between a hexahistidine tag (His(6)-tag) and DNA functionalized with three nitrilotriacetic acid (NTA) groups. The resulting DNA-protein linkage is site-specific. It can be broken quickly and controllably by the addition of a chelating agent that binds nickel. We have used this new linker to bind proteins to a variety of DNA motifs commonly used in the fabrication of nanostructures by DNA self-assembly.Erratum: Coordinated chemomechanical cycles: A mechanism for autonomous molecular motion (Physical Review Letters (2008) 101 (238101))
Physical Review Letters 102:13 (2009)
Kinetically controlled self-assembly of DNA oligomers.
J Am Chem Soc 131:7 (2009) 2422-2423
Abstract:
Metastable two-stranded DNA loops can be assembled into extended DNA oligomers by kinetically controlled self-assembly. Along the designed reaction pathway, the sequence of hybridization reactions is controlled by progressively revealing toeholds required to initiate strand-displacement reactions. The product length depends inversely on seed concentration and ranges from a few hundred to several thousand base-pairs.Algorithmic Control: The Assembly and Operation of DNA Nanostructures and Molecular Machinery
ALGORITHMIC BIOPROCESSES (2009) 215-225
Coordinated Chemomechanical Cycles: A Mechanism for Autonomous Molecular Motion (vol 101, 238101, 2008)
PHYSICAL REVIEW LETTERS 102:13 (2009) ARTN 139901