3P-133 Step detection of flagellar rotation at high temporal and spatial resolution(The 46th Annual Meeting of the Biophysical Society of Japan)
Seibutsu Butsuri Biophysical Society of Japan 48:supplement (2008) s148
ATP synthesis without R210 of subunit a in the Escherichia coli ATP synthase
Biochimica et Biophysica Acta - Bioenergetics 1777:1 (2008) 32-38
Characterization and Application of Controllable Local Chemical Changes Produced by Reagent Delivery from a Nanopipet.
J. Am. Chem. Soc. 130 (2008) 10387-10393
Abundance of Escherichia coli F1-ATPase molecules observed to rotate via single-molecule microscopy with gold nanorod probes.
J Bioenerg Biomembr 39:5-6 (2007) 435-439
Abstract:
The abundance of E. coli F1-ATPase molecules observed to rotate using gold nanorods attached to the gamma-subunit was quantitated. Individual F1 molecules were determined to be rotating based upon time dependent fluctuations of red and green light scattered from the nanorods when viewed through a polarizing filter. The average number of F1 molecules observed to rotate in the presence of GTP, ATP, and without nucleotide was approximately 50, approximately 25, and approximately 4% respectively. In some experiments, the fraction of molecules observed to rotate in the presence of GTP was as high as 65%. These data indicate that rotational measurements made using gold nanorods provide information of the F1-ATPase mechanism that is representative of the characteristics of the enzyme population as a whole.Recent developments of bio-molecular motors as on-chip devices using single molecule techniques.
Lab Chip 7:12 (2007) 1633-1643