Skip to main content
Home
Department Of Physics text logo
  • Research
    • Our research
    • Our research groups
    • Our research in action
    • Research funding support
    • Summer internships for undergraduates
  • Study
    • Undergraduates
    • Postgraduates
  • Engage
    • For alumni
    • For business
    • For schools
    • For the public
Menu
DNA tetrahedron

Professor Andrew Turberfield

Professor of Biological Physics

Research theme

  • Biological physics

Sub department

  • Condensed Matter Physics

Research groups

  • Nucleic acid nanotechnology
Andrew.Turberfield@physics.ox.ac.uk
  • About
  • Publications

Modifying Membrane Morphology and Interactions with DNA Origami Clathrin-Mimic Networks.

ACS nano (2019)

Authors:

Céline MA Journot, Vivek Ramakrishna, Mark I Wallace, Andrew J Turberfield

Abstract:

We describe the triggered assembly of a bio-inspired DNA origami meshwork on a lipid membrane. DNA triskelia, three-armed DNA origami nanostructures inspired by the membrane-modifying protein clathrin, are bound to lipid mono- and bi-layers using cholesterol anchors. Polymerization of triskelia, triggered by the addition of DNA staples, links triskelion arms to form a mesh. Using transmission electron microscopy, we observe nanoscale local deformation of a lipid monolayer induced by triskelion polymerization that is reminiscent of the formation of clathrin-coated pits. We also show that the polymerization of triskelia bound to lipid bilayers modifies interactions between them, inhibiting the formation of a synapse between giant unilamellar vesicles and a supported lipid bilayer.
More details from the publisher
Details from ORA
More details
More details

Peptide assembly directed and quantified using megadalton DNA nanostructures

ACS Nano American Chemical Society 13:9 (2019) 9927-9935

Authors:

Juan Jin, EG Baker, CW Wood, Jonathan Bath, DN Woolfson, Andrew Turberfield

Abstract:

In nature, co-assembly of polypeptides, nucleic acids, and polysaccharides is used to create functional supramolecular structures. Here, we show that DNA nanostructures can be used to template interactions between peptides and to enable the quantification of multivalent interactions that would otherwise not be observable. Our functional building blocks are peptide–oligonucleotide conjugates comprising de novo designed dimeric coiled-coil peptides covalently linked to oligonucleotide tags. These conjugates are incorporated in megadalton DNA origami nanostructures and direct nanostructure association through peptide–peptide interactions. Free and bound nanostructures can be counted directly from electron micrographs, allowing estimation of the dissociation constants of the peptides linking them. Results for a single peptide–peptide interaction are consistent with the measured solution-phase free energy; DNA nanostructures displaying multiple peptides allow the effects of polyvalency to be probed. This use of DNA nanostructures as identifiers allows the binding strengths of homo- and heterodimeric peptide combinations to be measured in a single experiment and gives access to dissociation constants that are too low to be quantified by conventional techniques. The work also demonstrates that hybrid biomolecules can be programmed to achieve spatial organization of complex synthetic biomolecular assemblies.

More details from the publisher
Details from ORA
More details
More details

Chiral DNA Origami Nanotubes with Well‐Defined and Addressable Inside and Outside Surfaces

Angewandte Chemie Wiley 130:26 (2018) 7813-7816

Authors:

Florence Benn, Natalie EC Haley, Alexandra E Lucas, Emma Silvester, Seham Helmi, Robert Schreiber, Jonathan Bath, Andrew J Turberfield
More details from the publisher

Dimensions and Global Twist of Single-Layer DNA Origami Measured by Small-Angle X-Ray Scattering.

ACS nano (2018)

Authors:

Matthew AB Baker, Andrew J Tuckwell, Jonathan F Berengut, Jonathan Bath, Florence Benn, Anthony P Duff, Andrew E Whitten, Katherine E Dunn, Robert M Hynson, Andrew J Turberfield, Lawrence K Lee

Abstract:

The rational design of complementary DNA sequences can be used to create nanostructures that self-assemble with nanometer precision. DNA nanostructures have been imaged by atomic force microscopy and electron microscopy. Small-angle X-ray scattering (SAXS) provides complementary structural information on the ensemble-averaged state of DNA nanostructures in solution. Here we demonstrate that SAXS can distinguish between different single-layer DNA origami tiles that look identical when immobilized on a mica surface and imaged with atomic force microscopy. We use SAXS to quantify the magnitude of global twist of DNA origami tiles with different crossover periodicities: these measurements highlight the extreme structural sensitivity of single-layer origami to the location of strand crossovers. We also use SAXS to quantify the distance between pairs of gold nanoparticles tethered to specific locations on a DNA origami tile and use this method to measure the overall dimensions and geometry of the DNA nanostructure in solution. Finally, we use indirect Fourier methods, which have long been used for the interpretation of SAXS data from biomolecules, to measure the distance between DNA helix pairs in a DNA origami nanotube. Together, these results provide important methodological advances in the use of SAXS to analyze DNA nanostructures in solution and insights into the structures of single-layer DNA origami.
More details from the publisher
Details from ORA
More details
More details

Chiral DNA origami nanotubes with well‐defined and addressable inside and outside surfaces

Angewandte Chemie International Edition Wiley‐VCH Verlag 57:26 (2018) 7687-7690

Authors:

F Benn, Natalie EC Haley, Alexandra E Lucas, Emma Silvester, Seham Helmi, R Schreiber, Jonathan Bath, Andrew J Turberfield

Abstract:

We report the design and assembly of chiral DNA nanotubes with well‐defined and addressable inside and outside surfaces. We demonstrate that the outside surface can be functionalised with a chiral arrangement of gold nanoparticles to create a plasmonic device and that the inside surface can be functionalised with a track for a molecular motor allowing transport of a cargo within the central cavity.
More details from the publisher
Details from ORA
More details
More details

Pagination

  • First page First
  • Previous page Prev
  • …
  • Page 2
  • Page 3
  • Page 4
  • Page 5
  • Current page 6
  • Page 7
  • Page 8
  • Page 9
  • Page 10
  • …
  • Next page Next
  • Last page Last

Footer Menu

  • Contact us
  • Giving to the Dept of Physics
  • Work with us
  • Media

User account menu

  • Log in

Follow us

FIND US

Clarendon Laboratory,

Parks Road,

Oxford,

OX1 3PU

CONTACT US

Tel: +44(0)1865272200

University of Oxfrod logo Department Of Physics text logo
IOP Juno Champion logo Athena Swan Silver Award logo

© University of Oxford - Department of Physics

Cookies | Privacy policy | Accessibility statement

Built by: Versantus

  • Home
  • Research
  • Study
  • Engage
  • Our people
  • News & Comment
  • Events
  • Our facilities & services
  • About us
  • Current students
  • Staff intranet