DNA-PAINT microscope data of a DNA nanostructure printer
University of Oxford (2022)
Abstract:
This dataset consist of reconstructed DNA-PAINT images of DNA origami based molecular devices. This is the data from the paper "A DNA molecular printer capable of programmable positioning and patterning in two dimensions". The data is structures after the figure of the paper. It is reconstructed and can be opened using the DNA-PAINT software Picasso. The data is described by what DNA paint probe was used to image it, corresponding to multiple image channels. 'P1' is the DNA-PAINT docking handle used on the frame and the canvas, 'R1' is the DNA-PAINT docking handle used on the sleeve, and 'R3' is the DNA-PAINT docking handle used on the ink patterned on the canvas.Reconfigurable T‐junction DNA origami
Angewandte Chemie International Edition Wiley 59:37 (2020) 15942-15946
Abstract:
DNA self‐assembly allows the construction of nanometre‐scale structures and devices. Structures with thousands of unique components are routinely assembled in good yield. Experimental progress has been rapid, based largely on empirical design rules. Here we demonstrate a DNA origami technique designed as a model system with which to explore the mechanism of assembly. The origami fold is controlled through single‐stranded loops embedded in a double‐stranded DNA template and is programmed by a set of double‐stranded linkers that specify pairwise interactions between loop sequences. Assembly is via T‐junctions formed by hybridization of single‐stranded overhangs on the linkers with the loops. The sequence of loops on the template and the set of interaction rules embodied in the linkers can be reconfigured with ease. We show that a set of just two interaction rules can be used to assemble simple T‐junction origami motifs and that assembly can be performed at room temperature.Chiral DNA Origami Nanotubes with Well‐Defined and Addressable Inside and Outside Surfaces
Angewandte Chemie Wiley 130:26 (2018) 7813-7816
Dimensions and Global Twist of Single-Layer DNA Origami Measured by Small-Angle X-Ray Scattering.
ACS nano (2018)