Condensed Matter Theory

Abstract:

During gastrulation, physical forces reshape the simple embryonic tissue to form the complex body plans of multicellular organisms¹. These forces often cause large-scale asymmetric movements of the embryonic tissue^2,3. In many embryos, the gastrulating tissue is surrounded by a rigid protective shell⁴. Although it is well-recognized that gastrulation movements depend on forces that are generated by tissue-intrinsic contractility^5,6, it is not known whether interactions between the tissue and the protective shell provide additional forces that affect gastrulation. Here we show that a particular part of the blastoderm tissue of the red flour beetle (Tribolium castaneum) tightly adheres in a temporally coordinated manner to the vitelline envelope that surrounds the embryo. This attachment generates an additional force that counteracts tissue-intrinsic contractile forces to create asymmetric tissue movements. This localized attachment depends on an αPS2 integrin (inflated), and the knockdown of this integrin leads to a gastrulation phenotype that is consistent with complete loss of attachment. Furthermore, analysis of another integrin (the αPS3 integrin, scab) in the fruit fly (Drosophila melanogaster) suggests that gastrulation in this organism also relies on adhesion between the blastoderm and the vitelline envelope. Our findings reveal a conserved mechanism through which the spatiotemporal pattern of tissue adhesion to the vitelline envelope provides controllable, counteracting forces that shape gastrulation movements in insects.

Abstract:

In this Letter, the sentence starting: 'For instance, Tribolium and Drosophila inflated are direct targets of the mesoderm…' has been corrected online; see accompanying Amendment.

Abstract:

We discuss a theoretical model of an on-demand single-particle emitter that employs a quantum dot, attached to an integer or fractional quantum Hall edge state. Via an exact mapping of the model onto the spin-boson problem we show that Coulomb interactions between the dot and the chiral quantum Hall edge state, unavoidable in this setting, lead to a destruction of precise charge quantization in the emitted wave-packet. Our findings cast doubts on the viability of this set-up as a single-particle source of quantized charge pulses. We further show how to use a spin-boson master equation approach to explicitly calculate the current pulse shape in this set-up.